the immunoassay handbook fourth edition theory and applications of ligand binding elisa and related techniques

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The Immunoassay Handbook

Author : David Wild
ISBN : 9780080970387
Genre : Science
File Size : 39. 4 MB
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The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing. www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com

Immunoassays

Author : Richard O'Kennedy
ISBN : 9781351797344
Genre : Medical
File Size : 28. 99 MB
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The concept behind this book is to provide a detailed and practical overview of the development and use of immunoassays in many different areas. Immunoassays are analytical tests that utilise antibodies to measure the amount, activity or identity of an analyte. This book is designed to provide a critical and helpful insight into the subject and to give the user practical information that may be of assistance in assay format selection, antibody generation/selection and choice of appropriate detection strategies. It is comprised of 13 chapters written by highly experienced researchers in the fields of antibody-based research, immunoassay development, assay validation, diagnostics and microfluidics. Beginning with a comprehensive survey of antibodies, immunoassay formats and signalling systems, the book elucidates key topics related to the development of an ideal antibody-based sensor, focuses on the important topic of surface modification, explores key parameters in the immobilisation of antibodies onto solid surfaces, discusses the move to ‘lab-on-a-chip’-based devices and investigates the key parameters necessary for their development. Three of the chapters are dedicated to the areas of clinical diagnostics, infectious disease monitoring and food security, where immunoassay-based applications have become highly valuable tools. The future of immunoassays, including next-generation immunoassays, electrochemical-immunoassays and ‘lab-on-a-chip’-based systems, is also discussed. The book also covers the use of optical detection systems (with a focus on surface plasmon resonance) in immunoassays, provides a compilation of important, routinely used immunoassay protocols and addresses problems that may be encountered during assay development.

Bioconjugate Techniques

Author : Greg T. Hermanson
ISBN : 9780123822406
Genre : Science
File Size : 87. 49 MB
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Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates Features full color illustrations Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Quantitative Human Physiology

Author : Joseph J Feher
ISBN : 9780128011546
Genre : Medical
File Size : 34. 16 MB
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Quantitative Human Physiology: An Introduction is the first text to meet the needs of the undergraduate bioengineering student who is being exposed to physiology for the first time, but requires a more analytical/quantitative approach. This book explores how component behavior produces system behavior in physiological systems. Through text explanation, figures, and equations, it provides the engineering student with a basic understanding of physiological principles with an emphasis on quantitative aspects. Features a quantitative approach that includes physical and chemical principles Provides a more integrated approach from first principles, integrating anatomy, molecular biology, biochemistry and physiology Includes clinical applications relevant to the biomedical engineering student (TENS, cochlear implants, blood substitutes, etc.) Integrates labs and problem sets to provide opportunities for practice and assessment throughout the course NEW FOR THE SECOND EDITION Expansion of many sections to include relevant information Addition of many new figures and re-drawing of other figures to update our understanding and clarify difficult areas Substantial updating of the text to reflect newer research results Addition of several new appendices including statistics, nomenclature of transport carriers, and structural biology of important items such as the neuromuscular junction and calcium release unit Addition of new problems within the problem sets Addition of commentary to power point presentations

Rna Methodologies

Author : Robert E. Farrell, Jr.
ISBN : 9780128046791
Genre : Science
File Size : 37. 42 MB
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RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals

The Protein Protocols Handbook

Author : John M. Walker
ISBN : 9781603272599
Genre : Science
File Size : 25. 56 MB
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In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Handbook Of Vitamins Third Edition

Author : Robert B. Rucker
ISBN : 1420029665
Genre : Technology & Engineering
File Size : 61. 13 MB
Format : PDF
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Features a comprehensive summary of the chemical, physiological, and nutritional relationships of all recognized vitamins! Maintaining the standards of excellence set forth in the previous editions, the Handbook of Vitamins, Third Edition presents a thorough examination of the fundamental characteristics, functions, and roles of vitamins in human health. Extensively updated and expanded to reflect the latest advances in analytical and separation methodologies! Offering a compendium of authoritative, current knowledge on the nature and function of each known nutrient, the Third Edition discusses.... improvements in the methodology, isolation, identification, and the synthesis of vitamins the chemistry, metabolism, and biochemical functions of vitamins vitamin interactions with environmental factors, drugs, alcohol, and smoking vitamins in disease prevention and health promotion the efficacy and hazards of high vitamin dosages and more! New sections cover... the roles vitamins play as catalysts, cellular regulators, and co-substrates biochemical markers for vitamin deficiency and groups at risk the relationship of B12 and folate metabolism to homocysteine regulation, and the possible connections of homocysteine to vascular diseases and developmental defects new roles for vitamins A, K, and D, and the role of vitamin E and flavonoids in oxidant defense Containing over 2800 literature references and 150 illustrations and tables, the Handbook of Vitamins, Third Edition serves as an indisputably valuable reference for human and animal nutritionists, dietitians, food scientists and technologists, biochemists, organic and analytical chemists, pharmacologists, toxicologists, physiologists, physicians in general practice, and makes an indispensable text for upper-level undergraduate and graduate students in these disciplines.

Molecular Biomethods Handbook

Author : John M. Walker
ISBN : 1603273751
Genre : Science
File Size : 52. 90 MB
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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Practical Enzymology

Author : Hans Bisswanger
ISBN : 9783527659241
Genre : Science
File Size : 31. 16 MB
Format : PDF, Kindle
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The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.

Lateral Flow Immunoassay

Author : Raphael Wong
ISBN : 1597452408
Genre : Science
File Size : 31. 13 MB
Format : PDF, ePub
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Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.

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