protein purification the basics garland science

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Protein Purification

Author : Philip Bonner
ISBN : 9780429856969
Genre : Medical
File Size : 48. 89 MB
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This second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification. Key Features * Guide to the major techniques used in protein purification * Includes flowcharts to help the reader slect the best purification strategy * Contains step-by-step protocols that guide the reader through each technique and its use * Includes exercises and solutions

Guide To Protein Purification

Author : Richard R Burgess
ISBN : 0080923178
Genre : Science
File Size : 61. 34 MB
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The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Molecular Biology Of The Cell

Author : Bruce Alberts
ISBN : 9781317563747
Genre : Science
File Size : 54. 90 MB
Format : PDF
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Culture Of Animal Cells

Author : R. Ian Freshney
ISBN : 9781118873373
Genre : Science
File Size : 28. 15 MB
Format : PDF
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Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

Measuring Gene Expression

Author : Matthew Avison
ISBN : 9781134189182
Genre : Science
File Size : 66. 83 MB
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In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.

Molecular Cell Biology

Author : Harvey Lodish
ISBN : 0716776014
Genre : Science
File Size : 55. 33 MB
Format : PDF, Kindle
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The sixth edition provides an authoritative and comprehensive vision of molecular biology today. It presents developments in cell birth, lineage and death, expanded coverage of signaling systems and of metabolism and movement of lipids.

Systems Biology And Synthetic Biology

Author : Pengcheng Fu
ISBN : 0470437979
Genre : Technology & Engineering
File Size : 20. 4 MB
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The genomic revolution has opened up systematic investigations and engineering designs for various life forms. Systems biology and synthetic biology are emerging as two complementary approaches, which embody the breakthrough in biology and invite application of engineering principles. Systems Biology and Synthetic Biology emphasizes the similarity between biology and engineering at the system level, which is important for applying systems and engineering theories to biology problems. This book demonstrates to students, researchers, and industry that systems biology relies on synthetic biology technologies to study biological systems, while synthetic biology depends on knowledge obtained from systems biology approaches.

Beginners Guide To Liquid Chromatography

Author : Waters Corporation
ISBN : 1879732025
Genre : Science
File Size : 66. 88 MB
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The Beginners Guide to Liquid Chromatography, a 56-page paperback book, is a useful tool for those learning about the technology of liquid chromatography (LC), with a focus on high-performance liquid chromatography (HPLC). The Beginners Guide offers an uncomplicated look at LC/HPLC and includes clear and colorful diagrams to acquaint the reader with basic concepts and terminology. No previous scientific training is required to obtain a better working knowledge of this powerful technology. Looking for something else? Learn a new technique or technology with the Waters Primers Series, view other titles available here: http://www.wiley.com/go/waters

Biotechnology Fundamentals

Author : Firdos Alam Khan
ISBN : 9781439820094
Genre : Medical
File Size : 89. 92 MB
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The focus of Biotechnology Fundamentals is to educate readers on both classical and modern aspects of biotechnology and to expose them to a range of topics, from basic information to complex technicalities. Other books cover subjects individually, but this text offers a rare topical combination of coverage, using numerous helpful illustrations to explore the information that students and researchers need to intelligently shape their careers. Keeping pace with the rapid advancement of the field, topics covered include: How biotechnology products are produced Differences between scientific research conducted in universities and industry Which areas of biotechnology offer the best and most challenging career opportunities Key laboratory techniques and protocols employed in the field The contents of this book are derived from discussions between teachers and undergraduate students and designed to address the concepts and methods thought useful by both sides. Starting with the fundamentals of biotechnology, coverage includes definitions, historical perspectives, timelines, and major discoveries, in addition to products, research and development, career prospects, ethical issues, and future trends. The author explains that even before it had been classified as its own field, biotechnology was already being applied in plant breeding, in vitro fertilization, alcohol fermentation, and other areas. He then delves into new developments in areas including stem cell research, cloning, biofuels, transgenic plants, genetically modified food/crops, pharmacogenomics, and nanobiotechnology. Incorporating extensive pedagogy into the content, this book provides plenty of examples, end-of-chapter problems, case studies, and lab tutorials to help reinforce understanding.

Animal Cell Culture And Technology

Author : Michael Butler
ISBN : 9781135325374
Genre : Science
File Size : 56. 27 MB
Format : PDF, ePub
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Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

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