immobilization of enzymes and cells methods in molecular biology

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Immobilization Of Enzymes And Cells

Author : Jose M. Guisan
ISBN : 9781597450539
Genre : Science
File Size : 54. 12 MB
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The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Immobilization Of Enzymes And Cells

Author : Jose M. Guisan
ISBN : 9781597450539
Genre : Science
File Size : 85. 88 MB
Format : PDF, Mobi
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The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Enzyme Stabilization And Immobilization

Author : Shelley D. Minteer
ISBN : 1493957929
Genre : Science
File Size : 87. 87 MB
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In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

Analytical Applications Of Immobilized Enzymes And Cells

Author : Lemuel B. Wingard
ISBN : 9781483215853
Genre : Science
File Size : 32. 73 MB
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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Techniques In Glycobiology

Author : R. Reid Townsend
ISBN : 0824798228
Genre : Science
File Size : 24. 95 MB
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This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.

Immobilization Of Enzymes And Cells

Author : Gordon Bickerstaff
ISBN : 0896033864
Genre : Science
File Size : 73. 69 MB
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Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.

Enzymes Of Molecular Biology

Author : Michael M. Burrell
ISBN : 0896033228
Genre : Medical
File Size : 84. 36 MB
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Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols.

Methods For General And Molecular Microbiology

Author : C. A. Reddy
ISBN : 9781555812232
Genre : Science
File Size : 43. 74 MB
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A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Immobilized Enzymes In Food And Microbial Processes

Author : Alfred Olson
ISBN : 9781468420883
Genre : Science
File Size : 36. 3 MB
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In the last five years the potential value of immobilized enzymes has captured the imagination of an increasing number of scientists and engineers. The concept of being able to create an immobilized derivative of an enzyme which has long-term stability and is able to be recovered and reused is fascinating, to say the least. Since the industrial application of enzymes has been mostly in the food and microbial process industry it is not surprising that many of the applications of immobilized enzymes considered for commercial development fall within the area of this industry. It is for this reason that we organized a symposium on immobilized enzymes for the 166th National Meeting of the American Chemical Society. Appropriately, the symposium was jointly sponsored by the Division of Agricultural and Food Chemistry and the Division of Microbial Chemistry and Technology. Although there were at least half a dozen symposia at other meetings on various aspects of immobilized enzyme technology in the preceding ten months none had specifically addressed themselves to food and microbial processes and none had been held at a meeting such as the National ACS gathering, which is not only large but multidisciplinary. The enthusiastic response to this symposium prompted us, at the invita tion of Plenum Press, to publish the proceedings of this symposium.

Fundamentals Of Cell Immobilisation Biotechnology

Author : Viktor Nedovic
ISBN : 9789401716383
Genre : Science
File Size : 63. 68 MB
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Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.

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